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opti mem medium  (Thermo Fisher)


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    Thermo Fisher opti mem medium
    Opti Mem Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opti-mem/Bafilomycin+A1/pm42363182-127-13-15
    Average 97 stars, based on 1 article reviews
    opti mem medium - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Incubation:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Transfection:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Purification:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Selection:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Cell Culture:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Gentle:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.

    Plasmid Preparation:

    Article Title: GLP-IR agonists and uses thereof
    Article Snippet: Briefly, plasmids encoding the GLP-1R-LgBiT and SmBiT-β-Arrestin2 fusions, and transfection reagent were diluted with Opti-MEM (Thermo Cat #31985-070).

    Article Title: CRISPR-Cas systems for genome editing
    Article Snippet: NLS-tagged Cas-alpha RNP complex was assembled by mixing 20 pmol of purified protein with 20 pmol of sgRNA in 25 μl Opti-MEM (Gibco) and incubated at room temperature for 30 min. After complex assembly 25 μl of Opti-MEM, containing 1.2 μl Lipofectamine 3000 (Thermo Fisher Scientific) was added and the mixture was incubated for additional 15 min at room temperature before transfection of the cells.

    Article Title: ARHGAP36 serves as a diagnostic and therapeutic marker that mediates immune escape and promotes thyroid cancer metastasis
    Article Snippet: Each siRNA was diluted in Opti-MEM (Thermo Fisher Scientific, Inc) to a final concentration of 100 nM in a 250 μl volume and incubated at room temperature for 5 min.

    Article Title: Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in mice
    Article Snippet: The mRNA and transfection reagent were initially diluted separately in Opti-MEM (51985091, Thermo Fisher, USA) and then incubated at room temperature for 5 min.

    Article Title: Multilayered molecular and cellular mechanisms underlying intestinal epithelial regeneration
    Article Snippet: After at least two days of selection, the cells were washed with DMEM without Zeocin and subsequently cultured in Advanced DMEM/F12 (12634010, Thermo Fisher Scientific) with 1% GlutaMax (35050-061, Gibco, Waltham, MA, USA) for 10 days to produce RSPO1 CM or Opti-MEM (31985070, Thermo Fisher Scientific) for 7 days to produce Noggin CM, respectively.

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: The transfection system comprised: dilution of 4 μl of Lipofectamine 2000 (Invitrogen) with 100 μl of Opti-MEM (Thermo Fisher Scientific) per sample well, inversion, gentle mixing, and incubation at RT for 5 min. Then, 1.6 ng of IFITM3 plasmid was diluted with 100 μl of Opti-MEM per sample well and cultured at RT for 5 min to form the transfection complex.

    Article Title: Genetic deletion or pharmacological inhibition of acetyl-CoA carboxylase 2 enhances fatty acid oxidation and improves cardiac function and survival in the murine ATGL knockout model of severe heart failure
    Article Snippet: RNP complex was made of crRNA:tracRNA du-plex (10 ng/μL final) and Cas9 Nuclease (10 ng/μL final) constituted in a total volume of 50 μL with Opti-MEM (31,985,070; Thermo Fisher Scientific, Waltham, MA) and used for electroporation.

    Article Title: CAKUT variants in PRPF8, DYRK2 , and CEP78 : implications for splicing and ciliogenesis
    Article Snippet: Mouse fibroblasts and RPE-1 cells were serum-starved in Opti-MEM (Thermo Fisher) for 24 hours, followed by smoothened agonist (SAG) treatment to induce Shh signaling.



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    opti mem  (MedChemExpress)
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    ( A ) Representative microscopic images showing the dose-dependent effects of 27-OHC and E2 on cell migration in HCT-116 cells after 48 h of treatment. Cells were exposed to four concentrations of 27-OHC (0.001, 0.01, 0.1, and 1 μM) and E2 (0.625, 1.25, 2.5, and 5 nM). Images were captured prior to the application of 1% SDS and reflect differences in cell migration across doses. ( B ) Quantitative analysis of 27-OHC-induced migration changes across four colon cancer cell lines. Cell migration was assessed using a trans-well assay following 48-h treatment with 27-OHC at the indicated concentrations. Experiments were performed in triplicate across three biological replicates ( n = 3). ( C ) Quantitative evaluation of E2-induced migration responses in colon cancer cells. Similarly, cell migration was assessed after 48 h of E2 treatment using the same four concentrations. Data were normalised to <t>phenol</t> <t>red-free</t> SFM controls and presented as fold changes relative to the untreated group. All graphs represent the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Dunnett's post hoc test.
    Opti Mem, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opti-mem/IBMX/pmc13170622-267-17-24
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    opti mem - by Bioz Stars, 2026-09
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    opti mem droplets  (Fisher Scientific)
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    ( A ) Representative microscopic images showing the dose-dependent effects of 27-OHC and E2 on cell migration in HCT-116 cells after 48 h of treatment. Cells were exposed to four concentrations of 27-OHC (0.001, 0.01, 0.1, and 1 μM) and E2 (0.625, 1.25, 2.5, and 5 nM). Images were captured prior to the application of 1% SDS and reflect differences in cell migration across doses. ( B ) Quantitative analysis of 27-OHC-induced migration changes across four colon cancer cell lines. Cell migration was assessed using a trans-well assay following 48-h treatment with 27-OHC at the indicated concentrations. Experiments were performed in triplicate across three biological replicates ( n = 3). ( C ) Quantitative evaluation of E2-induced migration responses in colon cancer cells. Similarly, cell migration was assessed after 48 h of E2 treatment using the same four concentrations. Data were normalised to <t>phenol</t> <t>red-free</t> SFM controls and presented as fold changes relative to the untreated group. All graphs represent the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Dunnett's post hoc test.
    Opti Mem Droplets, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opti-mem/mem+opti/pm42129543-238-31-33
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    phenol re free opti mem  (Thermo Fisher)
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    ( A ) Representative microscopic images showing the dose-dependent effects of 27-OHC and E2 on cell migration in HCT-116 cells after 48 h of treatment. Cells were exposed to four concentrations of 27-OHC (0.001, 0.01, 0.1, and 1 μM) and E2 (0.625, 1.25, 2.5, and 5 nM). Images were captured prior to the application of 1% SDS and reflect differences in cell migration across doses. ( B ) Quantitative analysis of 27-OHC-induced migration changes across four colon cancer cell lines. Cell migration was assessed using a trans-well assay following 48-h treatment with 27-OHC at the indicated concentrations. Experiments were performed in triplicate across three biological replicates ( n = 3). ( C ) Quantitative evaluation of E2-induced migration responses in colon cancer cells. Similarly, cell migration was assessed after 48 h of E2 treatment using the same four concentrations. Data were normalised to <t>phenol</t> <t>red-free</t> SFM controls and presented as fold changes relative to the untreated group. All graphs represent the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Dunnett's post hoc test.
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    Image Search Results


    ( A ) Representative microscopic images showing the dose-dependent effects of 27-OHC and E2 on cell migration in HCT-116 cells after 48 h of treatment. Cells were exposed to four concentrations of 27-OHC (0.001, 0.01, 0.1, and 1 μM) and E2 (0.625, 1.25, 2.5, and 5 nM). Images were captured prior to the application of 1% SDS and reflect differences in cell migration across doses. ( B ) Quantitative analysis of 27-OHC-induced migration changes across four colon cancer cell lines. Cell migration was assessed using a trans-well assay following 48-h treatment with 27-OHC at the indicated concentrations. Experiments were performed in triplicate across three biological replicates ( n = 3). ( C ) Quantitative evaluation of E2-induced migration responses in colon cancer cells. Similarly, cell migration was assessed after 48 h of E2 treatment using the same four concentrations. Data were normalised to phenol red-free SFM controls and presented as fold changes relative to the untreated group. All graphs represent the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Dunnett's post hoc test.

    Journal: Clinical Science (London, England : 1979)

    Article Title: Unravelling the effects of selective estrogen receptor modulators on colorectal cancer: a prognostic role for insulin-like growth factor binding protein-5

    doi: 10.1042/CS20258451

    Figure Lengend Snippet: ( A ) Representative microscopic images showing the dose-dependent effects of 27-OHC and E2 on cell migration in HCT-116 cells after 48 h of treatment. Cells were exposed to four concentrations of 27-OHC (0.001, 0.01, 0.1, and 1 μM) and E2 (0.625, 1.25, 2.5, and 5 nM). Images were captured prior to the application of 1% SDS and reflect differences in cell migration across doses. ( B ) Quantitative analysis of 27-OHC-induced migration changes across four colon cancer cell lines. Cell migration was assessed using a trans-well assay following 48-h treatment with 27-OHC at the indicated concentrations. Experiments were performed in triplicate across three biological replicates ( n = 3). ( C ) Quantitative evaluation of E2-induced migration responses in colon cancer cells. Similarly, cell migration was assessed after 48 h of E2 treatment using the same four concentrations. Data were normalised to phenol red-free SFM controls and presented as fold changes relative to the untreated group. All graphs represent the mean ± SD. Statistical significance was determined using one-way ANOVA followed by Dunnett's post hoc test.

    Article Snippet: HCT-116 and HT-29 cells (3 × 10 5 cells/well) were seeded into six-well plates and transfected with 50 nM siRNA (Dharmacon, ON-TARGET plus Human ESR2 (2100) siRNA-SMART pool, 20 nmol (L-003402-00-0020)) as performed previously [ ] using RNAiMAX Lipofectamine (Invitrogen) in phenol red-free Opti-MEM medium.

    Techniques: Migration

    Panels ( A , B ) illustrate the effect of varying concentrations of 27-OHC and E2 on the abundance of ERβ and IGFBP-5 in HCT-116 and HT-29 cell lines. The experiment was conducted in three biological repeats ( n = 3), with four different concentrations of 27-OHC (0.001, 0.1, 1, and 10 μM) or E2 (0.625, 1.25, 2.5, and 5 nM) used for treatment. The raw data from each Western blot experimental group were analysed through Image J and normalised against the phenol red-free SFM control group and were presented as fold changes relative to the control. Statistical analysis of differences was conducted using a one-way ANOVA with Dunnett's post hoc test showing with mean ± SD.

    Journal: Clinical Science (London, England : 1979)

    Article Title: Unravelling the effects of selective estrogen receptor modulators on colorectal cancer: a prognostic role for insulin-like growth factor binding protein-5

    doi: 10.1042/CS20258451

    Figure Lengend Snippet: Panels ( A , B ) illustrate the effect of varying concentrations of 27-OHC and E2 on the abundance of ERβ and IGFBP-5 in HCT-116 and HT-29 cell lines. The experiment was conducted in three biological repeats ( n = 3), with four different concentrations of 27-OHC (0.001, 0.1, 1, and 10 μM) or E2 (0.625, 1.25, 2.5, and 5 nM) used for treatment. The raw data from each Western blot experimental group were analysed through Image J and normalised against the phenol red-free SFM control group and were presented as fold changes relative to the control. Statistical analysis of differences was conducted using a one-way ANOVA with Dunnett's post hoc test showing with mean ± SD.

    Article Snippet: HCT-116 and HT-29 cells (3 × 10 5 cells/well) were seeded into six-well plates and transfected with 50 nM siRNA (Dharmacon, ON-TARGET plus Human ESR2 (2100) siRNA-SMART pool, 20 nmol (L-003402-00-0020)) as performed previously [ ] using RNAiMAX Lipofectamine (Invitrogen) in phenol red-free Opti-MEM medium.

    Techniques: Western Blot, Control

    ( A ) Effects of short-term exposure to E2 and 27-OHC on cell proliferation in HT-29 colon cancer cells. Cells were treated with E2 (5, 10, and 20 nM) or 27-OHC (0.1 and 1 μM) for 15 min, followed by incubation in phenol red-free SFM. Corresponding vehicle controls included phenol red-free SFM alone and ethanol at concentrations of 0.01% and 0.1%, matching the solvent concentrations used for 27-OHC. All experiments were performed in three independent biological replicates, each in technical triplicate ( n = 3). Proliferation data were normalised to the phenol red-free SFM control and presented as fold change. Statistical analysis was conducted using one-way ANOVA with Dunnett's post hoc test. Data are presented as mean ± SD. ( B ) Differential gene expression in SW620 cells treated with 10 nM E2 for 24 h based on mRNA sequencing data ( GSE112568 ). The graphs depict up-regulation of GPER1 and down-regulation of ZEB1 following E2 treatment. Each group was analysed in duplicate ( n = 2). ( C ) Intracellular Ca 2+ concentrations and cAMP levels measured in HCT-116 and HT-29 cells following 15-min treatment with E2 (10 nM) or 27-OHC (0.1 and 1 μM). Control groups received phenol red-free SFM or 0.1% ethanol, respectively. All data were normalised to the SFM control and presented as fold change. Experiments were conducted in three independent biological replicates ( n = 3), each in technical triplicate. Statistical comparisons were made using one-way ANOVA with Dunnett's post hoc test. Data are shown as mean ± SD. ( D ) Anti-proliferative effects of G1, a selective GPER1 agonist, following a 15-min exposure in HCT-116 and HT-29 cells. Cells were subsequently incubated in phenol red-free SFM for 48 h. Additionally, mRNA expression of GPER1 following short-term exposure to E2 and G1 was assessed. All experiments were performed in triplicate ( n = 3), and data were normalised to vehicle controls. Statistical significance was determined using one-way ANOVA with Dunnett's post hoc test. Data are expressed as mean ± SD. ( E ) GPER1 expression in CRC based on TCGA and GTEx datasets. E(i) Differential expression of GPER1 in colorectal tumour and normal tissues analysed via GEPIA2. For COAD, 275 tumour and 349 normal samples were included; for READ, 92 tumour and 318 normal samples were analysed. Gene expression is shown in log 2 (TPM + 1) units. Purple boxes represent normal tissues, and pink boxes represent tumour tissues. Statistical comparisons were performed using Student's t -test. E(ii) GPER1 expression levels in 438 TCGA colon cancer patients (204 females and 234 males), highlighting sex-specific expression differences. Data are shown as mean ± SD and analysed by Student's t -test. E(iii) Kaplan–Meier survival analysis of GPER1 and ERβ expression in 438 colon adenocarcinoma patients. Patients were stratified into high and low expression groups. The orange line denotes high expression and the blue line denotes low expression. Censored observations are indicated by plus signs (+). A steeper curve corresponds to lower survival probability. Statistical significance was P = 0.16 and P = 0.039.

    Journal: Clinical Science (London, England : 1979)

    Article Title: Unravelling the effects of selective estrogen receptor modulators on colorectal cancer: a prognostic role for insulin-like growth factor binding protein-5

    doi: 10.1042/CS20258451

    Figure Lengend Snippet: ( A ) Effects of short-term exposure to E2 and 27-OHC on cell proliferation in HT-29 colon cancer cells. Cells were treated with E2 (5, 10, and 20 nM) or 27-OHC (0.1 and 1 μM) for 15 min, followed by incubation in phenol red-free SFM. Corresponding vehicle controls included phenol red-free SFM alone and ethanol at concentrations of 0.01% and 0.1%, matching the solvent concentrations used for 27-OHC. All experiments were performed in three independent biological replicates, each in technical triplicate ( n = 3). Proliferation data were normalised to the phenol red-free SFM control and presented as fold change. Statistical analysis was conducted using one-way ANOVA with Dunnett's post hoc test. Data are presented as mean ± SD. ( B ) Differential gene expression in SW620 cells treated with 10 nM E2 for 24 h based on mRNA sequencing data ( GSE112568 ). The graphs depict up-regulation of GPER1 and down-regulation of ZEB1 following E2 treatment. Each group was analysed in duplicate ( n = 2). ( C ) Intracellular Ca 2+ concentrations and cAMP levels measured in HCT-116 and HT-29 cells following 15-min treatment with E2 (10 nM) or 27-OHC (0.1 and 1 μM). Control groups received phenol red-free SFM or 0.1% ethanol, respectively. All data were normalised to the SFM control and presented as fold change. Experiments were conducted in three independent biological replicates ( n = 3), each in technical triplicate. Statistical comparisons were made using one-way ANOVA with Dunnett's post hoc test. Data are shown as mean ± SD. ( D ) Anti-proliferative effects of G1, a selective GPER1 agonist, following a 15-min exposure in HCT-116 and HT-29 cells. Cells were subsequently incubated in phenol red-free SFM for 48 h. Additionally, mRNA expression of GPER1 following short-term exposure to E2 and G1 was assessed. All experiments were performed in triplicate ( n = 3), and data were normalised to vehicle controls. Statistical significance was determined using one-way ANOVA with Dunnett's post hoc test. Data are expressed as mean ± SD. ( E ) GPER1 expression in CRC based on TCGA and GTEx datasets. E(i) Differential expression of GPER1 in colorectal tumour and normal tissues analysed via GEPIA2. For COAD, 275 tumour and 349 normal samples were included; for READ, 92 tumour and 318 normal samples were analysed. Gene expression is shown in log 2 (TPM + 1) units. Purple boxes represent normal tissues, and pink boxes represent tumour tissues. Statistical comparisons were performed using Student's t -test. E(ii) GPER1 expression levels in 438 TCGA colon cancer patients (204 females and 234 males), highlighting sex-specific expression differences. Data are shown as mean ± SD and analysed by Student's t -test. E(iii) Kaplan–Meier survival analysis of GPER1 and ERβ expression in 438 colon adenocarcinoma patients. Patients were stratified into high and low expression groups. The orange line denotes high expression and the blue line denotes low expression. Censored observations are indicated by plus signs (+). A steeper curve corresponds to lower survival probability. Statistical significance was P = 0.16 and P = 0.039.

    Article Snippet: HCT-116 and HT-29 cells (3 × 10 5 cells/well) were seeded into six-well plates and transfected with 50 nM siRNA (Dharmacon, ON-TARGET plus Human ESR2 (2100) siRNA-SMART pool, 20 nmol (L-003402-00-0020)) as performed previously [ ] using RNAiMAX Lipofectamine (Invitrogen) in phenol red-free Opti-MEM medium.

    Techniques: Incubation, Solvent, Control, Gene Expression, Sequencing, Expressing, Quantitative Proteomics